HDAC5 modulates PD-L1 expression and cancer immunity via p65 deacetylation in pancreatic cancer

We performed HDAC5 knockdown in pancreatic cancer cell line (PANC-1). The expression profiling showed NF-κB signaling as a potential HDAC5 targets and the conclusions were futher validated in vivo and in vitro. RNA sequencing in PANC-1 cell lines with shControl or shHDAC5

本研究在胰腺癌细胞系PANC-1中开展了组蛋白去乙酰化酶5（HDAC5）的敲低实验。表达谱分析结果显示，核因子κB（NF-κB）信号通路为HDAC5的潜在靶标，且该结论已在体内与体外实验中得到进一步验证。本研究对转染了短发夹RNA对照（shControl）或短发夹RNA靶向HDAC5（shHDAC5）的PANC-1细胞系完成了RNA测序。