Expression data for eidididymal fat from high fat diet mice.

Using a C57BL6/J mouse model of diet-induced obesit,we observed that mannose supplementation of high fat diet (HFD)-fed mice prevents weight gain,lowers adiposity, reduces liver steatosis, and improves glucose tolerance and insulin sensitivity. We used microarrays to determine the gene expression pattern in epididymal fat frommice weaned on Normal diet (ND), HFD and HFD with mannose (HFDM) Epididymal fat was collected from 3 mice each weaned on ND or HFD or HFDM for RNA isolation.

本研究采用饮食诱导肥胖的C57BL6/J小鼠模型，发现向饲喂高脂肪饮食（high fat diet, HFD）的小鼠补充甘露糖，可抑制其体重增加、降低体脂率、减轻肝脏脂肪变性，并改善葡萄糖耐量与胰岛素敏感性。为明确附睾脂肪组织（epididymal fat）的基因表达谱，本研究使用基因芯片（microarrays），对断奶后分别饲喂正常饮食（Normal diet, ND）、HFD以及添加甘露糖的HFD（HFDM）的小鼠进行检测。实验中每组各选取3只小鼠采集附睾脂肪组织，用于RNA提取。