S. coelicolor YSK3654 R5- medium time course study

Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO3654::apr mutant strain (YSK3654) and compare it to the wild-type M145 strain. SCO3654 is a putative two-component system sensor kinase. The time-course study involved analysis of 9 samples (10h, 13h, 16h, 19h, 22h, 25h, 31h, 35h, 37h). All samples were analyzed on duplicate arrays (2 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

链霉菌（Streptomyces）属物种的抗生素生物合成过程，由全局调控因子与途径特异性调控因子共同构成的复杂遗传与生化网络所调控。目前，对于该网络中各调控因子介导次级代谢产物基因时空表达的精准互作机制，仍未得到充分阐明。本研究采用全基因组微阵列技术，对天蓝色链霉菌（Streptomyces coelicolor）A3(2) ΔSCO3654::apr突变菌株（YSK3654）的时序转录组谱进行分析，并与野生型M145菌株开展对比研究。SCO3654为推定的双组分系统感应激酶（two-component system sensor kinase）。本次时序实验共采集9个时间点的样本（10h、13h、16h、19h、22h、25h、31h、35h、37h），所有样本均进行双阵列重复分析（即2次技术重复）。本研究以天蓝色链霉菌野生型M145的基因组DNA作为所有微阵列实验的参照样本。互补DNA（complementary DNA，cDNA）采用Alexa647进行荧光标记，基因组DNA则以Cy3完成荧光标记。