Comprehensive analysis of the transcriptome-wide m6A methylation in mouse pachytene spermatocytes and round spermatids

Spermatogenesis, an efficient and complex system in male germline development, requires a series of elaborately regulated genetic events in which diploid spermatogonia differentiate into haploid spermatozoa. N6-methyladenosine (m6A) is important for spermatogenesis. ALKBH5 is an m6A eraser, and knockout of ALKBH5 increases the level of total m6A methylation and causes male infertility. comprehensive analyses of MeRIP-seq and RNA-seq data revealed differences between wild-type (WT) and Alkbh5 knockout (KO) mice.

精子发生（Spermatogenesis）是雄性生殖系发育中一套高效且复杂的系统，其过程需要一系列精密调控的遗传事件，二倍体精原细胞在此过程中分化为单倍体精子。N6-甲基腺嘌呤（m6A）对精子发生至关重要。ALKBH5作为一种m6A去甲基化酶，敲除该基因会提升总m6A甲基化水平，并导致雄性不育。对甲基化RNA免疫沉淀测序（MeRIP-seq）与RNA测序（RNA-seq）数据的综合分析，揭示了野生型（WT）与Alkbh5敲除（KO）小鼠之间的差异。