Gene expression profiling of prostate infiltrating hematopoietic cells in TRAMP mice subjected to immunotherapy. Mus musculus

Goal of the analysis was to identify the mechansisms accounting fo the synergy of T cells redirected to the tumor associated large T antigen and T cells redirected to the Uty minor histocompatibility antigen Overall design: Gene expression profiling on CD45+ cells isolated from the prostate of mice treated with Allo/Y, Tumor or Tumor+Allo/Y ACT at one week after T cell transfer, the time of acute prostate infiltration. Samples recovered from mice treated with the combined Tumor+Allo/Y ACT and sacrificed after 28 days were also included in the experimental design to take into account changes occurring over time. Three mice per group were used.

本分析的目标为阐明两类重定向T细胞的协同作用机制：一类是靶向肿瘤相关大T抗原（tumor associated large T antigen）的T细胞，另一类是靶向Uty次要组织相容性抗原（Uty minor histocompatibility antigen）的T细胞。实验设计方案如下：在T细胞输注后1周（即急性前列腺浸润发生的时间点），对经Allo/Y、Tumor或Tumor+Allo/Y ACT处理的小鼠前列腺中分离得到的CD45阳性细胞(CD45+ cells)进行基因表达谱分析。此外，本实验还纳入了经联合Tumor+Allo/Y ACT处理、并于28天后处死的小鼠样本，以考察随时间推移出现的表达变化。每组使用3只小鼠开展实验。