Evalutation of the effect of ethanol upon gene expression in adult Drosophila melanogaster. Drosophila melanogaster

This experiment was intended to determine if topical exposure to ethanol led to significant changes in gene expression in adult Drosophila melanogaster. It was determined that topical exposure to ethanol did not lead to a strong differential expression of genes in Drosophila after 4 hours. Keywords: Ethanol response, stress response Overall design: The effect of topical treatment with 99% ethanol was compared to the effect of a water treatment which was used as a control and the differential expression between these two treatments was assessed four hours after treatment. Two biological replicates of each treatment were compared for a total of two microarray hybridizations

本实验旨在探究表皮接触乙醇是否会导致成年黑腹果蝇（Drosophila melanogaster）的基因表达发生显著变化。经检测，表皮接触乙醇4小时后，果蝇体内未出现显著的基因差异表达。
关键词：乙醇应答、应激应答
实验设计：将99%乙醇表皮处理的效果与以水处理作为对照的处理效果进行对比，并在处理后4小时评估两组处理间的基因差异表达情况。每种处理设置两个生物学重复，共计完成两次微阵列杂交实验。