Mean C_t values derived from real time RT-PCR analyses of nucleic acid extracts prepared by different extraction methods using the blood, stool and sputum test panels.

Shaded and non-shaded rows represent spiked (positive) and non-spiked (negative) samples, respectively; A–F represent coded identifiers of the six developers; the CDC used the Roche MagNaPure platform; PATH used the Qiagen extraction kits except for MTB; SP, sputum; BL, blood; ST, stool; ND no amplification detected; a 1–2 false negative RT-PCR replicate(s) detected within a confirmed positive member; b false positive RT-PCR replicate(s) detected within negative sample; Sens, mean sensitivity across all nine replicates; Spec, mean specificity across all nine replicates; CI, confidence interval. Dark shading indicates spiked samples; NC, not calculated.带阴影与无阴影的行分别代表加标（阳性）与未加标（阴性）样本；A~F为六位开发者的编码标识符。美国疾病控制与预防中心（CDC）采用罗氏MagNaPure平台；PATH除结核分枝杆菌（MTB）外，均使用凯杰（Qiagen）提取试剂盒。SP代表痰液（sputum），BL代表血液，ST代表粪便；ND表示未检测到扩增。a：在确认阳性样本中检测到1~2次逆转录聚合酶链式反应（RT-PCR）重复检测结果为假阴性；b：在阴性样本中检测到假阳性RT-PCR重复检测结果。Sens为全部9次重复实验的平均灵敏度；Spec为全部9次重复实验的平均特异度；CI为置信区间。深阴影代表加标样本；NC表示未计算。