Raw proteomics data of endosomes isolated from mouse brain endothelial cells

We sought to determine whether sleep influences the endosomal protein profile of brain endothelial cells. One group of mice was subjected to 6 h of sleep deprivation (SD) beginning at Zeitgeber Time 0 (ZT0; lights on), followed by 2 h of recovery sleep, whereas a second group underwent 8 h of continuous SD. Brain endothelial cells were isolated using CD31 antibody–conjugated beads, and endosomes purified from these cells were analyzed by LC–MS/MS–based proteomics. To assess whether comparable alterations occur across the natural sleep–wake cycle, brain endothelial cells were also collected at two circadian time points, ZT8 (sleep phase) and ZT20 (wake phase). Endosomes isolated from these samples were likewise subjected to LC–MS/MS proteomic analysis. The original mass spectrometry raw data are provided here.本研究旨在探究睡眠是否会影响脑内皮细胞的内体蛋白质谱。将小鼠分为两组：一组从授时因子时间0（Zeitgeber Time 0，ZT0；光照开启时刻）开始接受6小时睡眠剥夺（sleep deprivation，SD），随后给予2小时恢复睡眠；另一组则接受连续8小时的睡眠剥夺。采用CD31抗体偶联微珠分离脑内皮细胞，对从中纯化得到的内体进行基于液相色谱-串联质谱（LC–MS/MS）的蛋白质组学分析。 为评估自然睡眠-觉醒周期中是否存在类似的蛋白表达变化，研究人员还在两个昼夜时间点采集了脑内皮细胞，分别为ZT8（睡眠时段）与ZT20（觉醒时段）。对从这些样本中分离得到的内体同样开展LC–MS/MS蛋白质组学分析。本数据集提供原始质谱数据。