RNA-seq of gastrocnemius (GA) muscle tissue from mice treated with extracellular vesicles (EV) from MCF-10A or MDA-MB-231 cells

To compare gene expression changes in skeletal muscle caused by EVs from normal (MCF-10A) and cancer (MDA-MB-231) cells, we analyzed RNA isolated from the GA muscle of EV-treated female NOD scid gamma (NSG) mice. Mice had received tail-vein injections of EVs twice a week for 5 weeks (~10 ug EV per injection). Gene expression in muscle from mice treated with MDA-MB-231-derived EVs was compared to mice treated with MCF-10A-derived EVs. RNA-seq was used for transcriptomic profiling of GA muscle following a total of 10 times of intravenous EV injections (MCF-10A EVs or MDA-MB-231 EVs) in NSG mice. RNA-seq and data processing were performed by Novogene Co., Ltd.

为比较正常细胞（MCF-10A）与癌细胞（MDA-MB-231）来源的细胞外囊泡（Extracellular Vesicles, EVs）所诱导的骨骼肌基因表达变化，我们对经EV处理的雌性非肥胖糖尿病重症联合免疫缺陷γ（NOD scid gamma, NSG）小鼠的腓肠肌（GA muscle）中分离得到的RNA进行了分析。小鼠每周接受两次尾静脉EV注射，共持续5周，单次注射剂量约为10微克EV。本研究将注射MDA-MB-231来源EV的小鼠肌肉组织的基因表达水平，与注射MCF-10A来源EV的小鼠的对应指标进行对照。在对NSG小鼠完成总计10次静脉注射EV（MCF-10A来源EV或MDA-MB-231来源EV）后，通过RNA测序（RNA-seq）对其腓肠肌开展转录组分析。RNA测序及数据处理工作由诺禾致源（Novogene Co., Ltd.）完成。