L3-L4_reference_tiling_array

modENCODE_submission_659 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: N2; Tissue: reference (L3-L4); Developmental Stage: L3-L4 larva 20dC 22h 23dC 24hr post-L1; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Antibody EZview Red ANTI-FLAG® M2 Affinity Gel (target is FLAG); Strain N2; temperature 23; Developmental Stage L3-L4 larva 20dC 22h 23dC 24hr post-L1; Tissue reference (L3-L4)

modENCODE_submission_659 本次提交源自Robert Waterston的modENCODE项目。modENCODE项目完整列表可参阅：http://www.genome.gov/26524648。

项目目标：本实验通过将RNA与商用基因组平铺阵列（genome tiling arrays）杂交，以检测秀丽隐杆线虫（C. elegans）的全部转录本。为最大化检测特定细胞中低表达稀有转录本的概率，我们分别从经荧光激活细胞分选（FACS）分离的特异性胚胎细胞，以及采用mRNA标记法获取的胚后细胞中提取RNA。

数据使用条款与规范请参阅：http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。

关键词：转录本平铺阵列分析（Transcript tiling array analysis）

实验类型：转录本平铺阵列分析。

生物来源：菌株：N2；组织：参照组（L3-L4期）；发育阶段：L3-L4期幼虫，L1蜕皮后于20℃培养22小时、23℃培养24小时；基因型：野生型；性别：雌雄同体。

重复次数：3次。

实验因素：抗体：EZview Red 抗FLAG® M2亲和凝胶（靶标为FLAG标签）；菌株：N2；温度：23℃；发育阶段：L3-L4期幼虫，L1蜕皮后于20℃培养22小时、23℃培养24小时；组织：参照组（L3-L4期）。