Transcriptomic analysis of Drosophila larval and adult blood cells

Drosophila adult blood cells originate from larval peripheral hemocytes (derived from the embryonic waves of hematopoiesis) and from larval lymph gland hemocytes. To gain insight into the expression repertoire of Drosophila blood cells, we established the gene expression profiles the adult blood cells and their ascendants. Overall design: We used Drosophila melanogaster w1118 females to retrieve total blood cell populations. Adult blood cells were collected by bleeding 5-day old flies; 3rd instar larval hemocytes were retrieved by bleeding (for embryo-derived hemocytes) or by dissecting the anterior lobes of the lymph glands.Total RNAs were extracted for analysis by deep sequencing, in triplicate, using Illumina NextSeq sequencing platform.

果蝇成体血细胞起源于幼虫外周血细胞（源自胚胎造血波）以及幼虫淋巴腺血细胞。为深入探究果蝇血细胞的基因表达谱全貌，我们构建了成体血细胞及其上游前体细胞的基因表达谱。实验设计：本研究以黑腹果蝇（Drosophila melanogaster）w1118品系的雌虫为实验材料，分离获取总血细胞群。通过刺破5日龄成虫采血获取成体血细胞；针对三龄幼虫血细胞，胚胎来源的幼虫血细胞可通过采血分离，而淋巴腺来源的幼虫血细胞则通过解剖淋巴腺前叶获取。随后提取总RNA，采用Illumina NextSeq测序平台进行三次生物学重复的深度测序分析。