Real-time quantitative PCR analysis of spleen cells

TGF-β1 signaling pathway of spleen of the Gata1low mouse model of myelofibrosis Four condition experiment. Biological replicates: 3 control CD1 mice, 3 Gata1low mice, 3 SB431542-treated Gata1low mice and 3 Vehicle-treated Gata1low mice.本实验针对骨髓纤维化Gata1low小鼠模型的脾脏转化生长因子-β1（TGF-β1）信号通路开展四组条件实验。生物学重复设置如下：3只对照CD1小鼠、3只Gata1low小鼠、3只经SB431542处理的Gata1low小鼠，以及3只经溶剂（Vehicle）处理的Gata1low小鼠。