Additional file 2 of Transcriptome analysis of the transdifferentiation of canine BMSCs into insulin producing cells

Additional file 2. A comparison of mapped reads and the reference genome alignment. “Total mapped” --- The number of reads that can be mapped to the genome. “Uniquely mapped” --- The number of reads with a unique matched alignment position on the reference sequence. “Multiple mapped” --- The number of reads with multipul matched alignment positions on the reference sequence. “Reads map to ‘+’” --- The reads that can map to the sense strand of genome. “Reads map to ‘-’” --- The reads that can map to the antisense strand of genome. “Non-splice reads” --- The number of the intact Uniquely mapped reads which map to exons. “Splice reads” --- The number of the segmented Uniquely mapped reads which map to exons. Our samples owned relatively high quality with over 90% mapped clean reads.附加文件2：比对读段与参考基因组比对结果的对比分析。"总比对读段（Total mapped）"：指可比对至参考基因组的测序读段数量。"唯一比对读段（Uniquely mapped）"：指在参考序列上仅存在单一匹配比对位置的测序读段数量。"多位置比对读段（Multiple mapped）"：指在参考序列上存在多个匹配比对位置的测序读段数量。"正义链比对读段（Reads map to '+'）"：指可比对至基因组正义链的测序读段。"反义链比对读段（Reads map to '-'）"：指可比对至基因组反义链的测序读段。"非剪接读段（Non-splice reads）"：指完整匹配至外显子（exon）的唯一比对读段数量。"剪接读段（Splice reads）"：指分段匹配至外显子的唯一比对读段数量。本研究样本整体质量较高，比对上的洁净读段（clean reads）占比超过90%。