hMTR4 plays a central role in creating balanced nuclear RNA pools for degradation and export V. Homo sapiens

To examine whether the competition between hMTR4 with AlyREF is important for the specific exosome recruitment,we sequenced the RNAs associating with hMTR4 in control and AlyREF overexpression cells. Overall design: hMTR4 associated RNAs isolated from control or AlyREF overexpression HeLa cells were generated by deep sequencing, using Illumina HiSeq X Ten.

为探究hMTR4与AlyREF之间的竞争对特异性外泌体（exosome）招募是否具有重要意义，我们对对照组与过表达AlyREF的细胞中与hMTR4结合的RNA进行了测序。整体实验设计：从对照组或过表达AlyREF的HeLa细胞中分离得到的与hMTR4结合的RNA，采用Illumina HiSeq X Ten平台进行深度测序。