Small RNA sequencing in wild chickpea

We report small RNA data from the leaves of wild chickpea PI 489777. Small RNA library was prepared and sequencing was performed using Illumina platform. A total of 23 million reads were generated, which represented 0.95 million unique reads. These were mapped to the chickpea genome using Bowtie to obtain the non-redundant set of unique small RNA sequences. In this study, RNA was isolated from the leaves of PI 489777 and small RNA library was prepared using TruSeq Small RNA Sample Prep Kit (Illumina Technologies) according to the manufacturer's instructions. Data obtained in FASTQ files was pre-processed to remove low-quality reads. Unique reads from each sample were mapped to the chickpea genome using Bowtie.Only the non-redundant set of uniquely mapped small RNA reads were retained.

本研究报道了野生鹰嘴豆（wild chickpea）PI 489777叶片的小RNA（small RNA）数据。本研究采用Illumina测序平台完成小RNA文库的构建与测序，共生成2300万条测序读段（reads），其中包含95万条唯一读段。研究人员利用Bowtie软件将上述读段比对至鹰嘴豆基因组，以获取唯一小RNA序列的非冗余集合。在本研究中，实验人员从PI 489777的叶片中提取总RNA，并按照制造商说明书，采用TruSeq Small RNA Sample Prep Kit（Illumina Technologies）构建小RNA文库。所得FASTQ格式数据经预处理以去除低质量测序读段；将每个样本中的唯一读段利用Bowtie比对至鹰嘴豆基因组，最终仅保留唯一比对成功的非冗余小RNA读段集合。