Transgenic Mouse Enhancer Assay - Vista Enhancer ID: mm458

See link to the data preparation protocols for this study in the Additional Information section See links to Genome Browser in the Additional Information section. **Enhancer location:** Mouse genome mm9, chr15:63025492-63026341 **Name:** mCF144 **Vista Enhancer ID:** mm458 **Nearest craniofacial gene or locus:** NOG **Enhancer expression:** positive **Tissues where active:** branchial arch, limbs, other **Comment on OPT files:** no clip file This data set includes the following: * A .pdf file that summarizes the whole-mount data for the tested element. This file includes light microscopy whole-mount images of the LacZ reporter gene expression in mouse embryos, a summary of the annotation of the expression pattern, the DNA sequence of the tested element in Fasta format, and the sequences of the primers used to generate the test construct. Follow corresponding link in the Additional Information section to see this information in the Vista Enhancer Browser at Lawrence Berkeley Laboratory * 3 movie (.avi) files: 3 movies representing a pass-through view of the OPT model in the coronal, sagittal, or transverse plane. See links in External Referece section to download the following movie files: Coronal plane movie, Sagittal plane movie, Transverse plane movie * 3 files in .tgz format. Each of these corresponds to one of the .avi files and unzips to a set of .png files, each of which represents the individual frames of the movie (and, in case of the transverse/sagittal/coronal movies, can be used to retrieve individual virtual sections through features of interest in .png image format). [.tgz files can be unzipped with WinZip or Stuffit on a PC or Mac. On linux, treat the file as a gzipped .tar file: gunzip < file.tgz | tar xvf - ] * Tested element.txt: Download the text file mCF144metadata.txt from the External Reference section .

本研究的数据制备方案链接及基因组浏览器链接，请参见【附加信息】部分。**增强子（Enhancer）位点**：小鼠基因组mm9版本，15号染色体：63025492-63026341 **名称**：mCF144 **Vista增强子ID（Vista Enhancer ID）**：mm458 **最近的颅面基因或基因座**：NOG **增强子表达**：阳性 **活跃组织**：鳃弓、肢体及其他组织 **OPT文件说明**：无剪辑文件

本数据集包含以下内容：
* 一份.pdf格式文件：汇总了待测元件的整体原位杂交数据，内含小鼠胚胎中LacZ报告基因表达的光学显微镜整体成像图、表达模式注释摘要、Fasta格式的待测元件DNA序列，以及用于构建测试载体的引物序列。如需查看劳伦斯伯克利实验室（Lawrence Berkeley Laboratory）的Vista增强子浏览器（Vista Enhancer Browser）中的此类信息，请访问【附加信息】中的对应链接。
* 3个.avi格式视频文件：分别展示OPT模型在冠状面、矢状面和横断面的穿透视图。如需下载这些视频文件，请参见【外部参考】部分的对应链接：冠状面视频、矢状面视频、横断面视频。
* 3个.tgz格式压缩包：每个压缩包对应上述一个.avi视频文件，解压后可得到一组.png格式图片，每张图片对应视频的单帧画面（对于冠状、矢状或横断面视频，可用于提取目标特征的虚拟切片png图像）。【.tgz文件解压方法：在PC或Mac端可使用WinZip或Stuffit工具；在Linux系统中，可将其视为gzip压缩的tar文件，执行命令`gunzip < file.tgz | tar xvf -`进行解压。】
* 待测元件文本文件：请从【外部参考】部分下载文本文件mCF144metadata.txt。