Recombination analysis raw data

This research delves into the understanding of the formation of recombinant forms between HIV-1 and HIV-2. Since both HIV-1 and HIV-2 infect the same cell type, they can certainly reside in the same cell and undergo recombination. Recombination between HIV-1 and HIV-2 might have important ramifications for the disease progression phenotype observed in HIV-1 and HIV-2 co-infected individuals. We developed a bioinformatics analysis pipeline which performs an in-depth analysis of recombination to detect these recombinant forms. We found HIV-1/HIV-2 recombinants in circulation based on our analysis. Thus, we conclude that there are HIV-1/HIV-2 recombinants in circulation, and these recombinants are likely to contribute to the observed slow progression of disease in dually infected individuals. Our analysis pipeline is reproducible and can be adopted for the recombination analysis of any retrovirus.

本研究旨在深入解析人类免疫缺陷病毒1型（HIV-1）与人类免疫缺陷病毒2型（HIV-2）之间重组体的形成机制。由于HIV-1与HIV-2均可感染同一类细胞，二者完全可以共存于同一宿主细胞并发生重组事件。HIV-1与HIV-2之间的重组，可能对共感染个体所观察到的疾病进展表型产生重要影响。本研究开发了一套生物信息学分析流程（bioinformatics analysis pipeline），可对重组事件进行深度分析以识别此类重组体。通过本分析流程，我们在流行毒株中检测到了HIV-1/HIV-2重组体。据此我们得出结论：当前流行的毒株中确实存在HIV-1/HIV-2重组体，且此类重组体可能是共感染个体出现疾病缓慢进展表型的相关因素之一。本研究所用的分析流程具备可重复性，可推广应用于任意逆转录病毒（retrovirus）的重组事件分析。