File S1 - Targeting NAD⁺ Metabolism in the Human Malaria Parasite <i>Plasmodium falciparum</i>

Contains the files: Figure S1. PfNico (PFC0910w) localization throughout the IDC. Live imaging of episomally expressed enzyme-GFP fusion proteins in the parasite. Figure S2. Labeling pattern of NAD+. The resulting labeling pattern of NAD+ from C13-U-glucose is depicted. Figure S3. Observed labeling pattern of NAD+ in iRBCs. Comparison of half and full labeled NAD+ generated in iRBCs in the presence of C13-U-glucose. Figure S4. NAD+ synthesis under different niacin conditions. Observed fold change in NAD+ concentration resulting from different niacin present in the culture medium. Figure S5. Alignment of PfNMNAT and the E. coli homolog NadD. Comparison of the amino acid sequences of the parasite and bacterial NMNAT enzymes. Figure S6. Complementation of E. coli NadD with PfNMNAT. The ability of the parasite NMNAT to rescue E. coli growth is demonstrated in a continuous growth assay. Figure S7. Alignment of PfNMNAT and the Human NMNAT homologs. Comparison of the amino acid sequences of the parasite and human NMNAT enzymes. Table S1. Primers Used in This Study. Table S2. Strains Used in This Study. (PDF)本数据集包含以下文件： 补充图S1：恶性疟原虫PfNico（基因编号PFC0910w）在红细胞内发育周期（Intraerythrocytic Developmental Cycle, IDC）中的定位：对该寄生虫体内游离表达的酶-绿色荧光蛋白（Green Fluorescent Protein, GFP）融合蛋白开展活细胞成像所获结果。 补充图S2：烟酰胺腺嘌呤二核苷酸（NAD+）标记模式：展示了以C13-U-葡萄糖为底物标记得到的NAD+的标记图谱。 补充图S3：感染红细胞（infected red blood cells, iRBCs）内NAD+的实际标记模式：对比了在C13-U-葡萄糖存在条件下于感染红细胞中生成的半标记与全标记NAD+。 补充图S4：不同烟酸条件下的NAD+合成：展示了培养基中添加不同浓度烟酸时，NAD+浓度的相对变化倍数。 补充图S5：PfNMNAT与大肠杆菌（Escherichia coli, E. coli）同源蛋白NadD的序列比对：对比了疟原虫与细菌NMNAT酶的氨基酸序列。 补充图S6：利用PfNMNAT互补大肠杆菌NadD缺陷株：通过持续生长实验验证了疟原虫NMNAT能够恢复大肠杆菌的生长能力。 补充图S7：PfNMNAT与人类NMNAT同源蛋白的序列比对：对比了疟原虫与人类NMNAT酶的氨基酸序列。 补充表S1：本研究使用的引物序列。 补充表S2：本研究使用的菌株信息。 （PDF格式）