RNA seq analysis of Nfe2 expressing 4T1 and TS/A cells

We founed enhanced Nfe2 mRNA expression in bone tropic mouse breast cancer cell lines, 4T1.3, grown in a bone cavity, compared with in vitro culture condition. Thus, we examined about the function of Nfe2 in tumor growth in bone microenvironment in this study. Overall design: Total RNA was extracted from mouse breast cancer cell lines 4T1 and TS/A cells which wre transduced Nfe2 gene. The resultant total RNAs were subjected to RNAseq.我们发现，相较于体外培养条件，在骨腔中生长的亲骨性小鼠乳腺癌细胞系4T1.3内，Nfe2的mRNA（messenger RNA）表达水平显著升高。因此，本研究旨在探究Nfe2在骨微环境中对肿瘤生长的调控作用。实验整体设计：从转导了Nfe2基因的小鼠乳腺癌细胞系4T1与TS/A中提取总RNA（Total RNA），所获得的总RNA随后进行RNA测序（RNAseq）。