RNA-seq of primary human macrophages infected with Yersinia enterocolitica strains. RNA-seq of primary human macrophages infected with Yersinia enterocolitica strains

Pathogenic bacteria Yersinia enterocolitica injects virulence plasmid-encoded effectors through the type three secretion system into macrophages to modulate gene expression. Here we analyzed the effect on gene expression in primary human macrophages of Y. enterocolitica strains lacking effector YopP (1.5 h infection) or effectors YopP and YopM (1.5 h or 6 h infection) simultaneously using RNA-seq. This is part of a larger sequencing experiment for which other samples can be found in EMBL-EBI (www.ebi.ac.uk/arrayexpress) under accession number E-MTAB-10473 and European Nucleotide Archive (ENA) at http://www.ebi.ac.uk/ena/data/view/PRJEB10086.

致病性细菌小肠结肠炎耶尔森氏菌（Yersinia enterocolitica）可通过三型分泌系统（type three secretion system）将毒力质粒编码的效应蛋白注入巨噬细胞，以调控宿主基因表达。本研究利用RNA测序（RNA-seq）技术，分析了感染缺失效应蛋白YopP（感染1.5小时）、或同时缺失效应蛋白YopP与YopM（感染1.5小时或6小时）的小肠结肠炎耶尔森氏菌菌株对原代人巨噬细胞基因表达的影响。该数据集属于一项更大规模测序实验的组成部分，其余样本可在欧洲分子生物学实验室-欧洲生物信息研究所（EMBL-EBI，www.ebi.ac.uk/arrayexpress）获取，对应登录号为E-MTAB-10473；相关数据亦可通过欧洲核苷酸档案库（ENA，http://www.ebi.ac.uk/ena/data/view/PRJEB10086）查询。