Detection of chromosomal aberrations in renal tumors by virtual karyotyping with SNP microarrays. Homo sapiens

Renal epithelial neoplasms have characteristic chromosomal imbalances that can be used for classification. We have previously shown that virtual karyotypes (v-karyotype) derived from SNP microarrays can be performed on formalin-fixed paraffin embedded (FFPE) tissue samples but a direct comparison with karyotypes obtained by conventional cytogenetics has not been done. 20 archival FFPE tumor samples were analyzed with Affymetrix 10K 2.0 or 250K Nsp SNP microarrays. Overall design: 19 archival FFPE tumor samples were analyzed with Affymetrix 10K 2.0 or 250K Nsp SNP microarrays and virtual-karyotype results compared to those obtained by Cytogenetics.

肾上皮肿瘤（Renal epithelial neoplasms）具备可用于分类的特征性染色体失衡（chromosomal imbalances）。本团队既往研究证实，基于单核苷酸多态性微阵列（SNP microarrays）得到的虚拟核型（virtual karyotypes，简称v-karyotype）可应用于福尔马林固定石蜡包埋（FFPE）组织样本，但尚未开展该虚拟核型与常规细胞遗传学（conventional cytogenetics）所得核型的直接对比研究。本研究采用Affymetrix 10K 2.0或250K Nsp单核苷酸多态性微阵列，对20份存档福尔马林固定石蜡包埋肿瘤样本进行分析。实验整体设计：采用Affymetrix 10K 2.0或250K Nsp单核苷酸多态性微阵列，对19份存档福尔马林固定石蜡包埋肿瘤样本进行分析，并将所得虚拟核型结果与细胞遗传学检测结果进行对比。