Expression profiling of IL-13 stimulated PBMCs with and without an IL-13R antagonist

This experiment aims to identify the biological pathways and diseases associated with the cytokine Interleukin 13 (IL-13) using gene expression measured in peripheral blood mononuclear cells (PBMCs). Overall design: The experiment comprised of samples obtained from 3 healthy donors. The expression profiles of in vitro IL-13 stimulation were generated using RNA-seq technology for 3 PBMC samples at 24 hours. The transcriptional profiles of PBMCs without IL-13 stimulation were also generated to be used as controls. An IL-13R-alpha antagonist (Redpath et al. Biochemical Journal, 2013) was introduced into IL-13 stimulated PBMCs and the gene expression levels after 24h were profiled to examine the neutralization of IL-13 signaling by the antagonist.本实验旨在利用外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）中测得的基因表达量，鉴定与细胞因子白细胞介素13（Interleukin 13, IL-13）相关的生物学通路与疾病。总体设计：本实验共采集3名健康供者的样本。通过RNA-seq技术，对经体外IL-13刺激24小时的3份PBMC样本生成表达谱；同时设置未接受IL-13刺激的PBMC样本作为对照。本实验还向经IL-13刺激的PBMC样本中加入白细胞介素13受体α拮抗剂（IL-13R-alpha antagonist，Redpath等，《Biochemical Journal》，2013），并在24小时后检测其基因表达水平，以考察该拮抗剂对IL-13信号通路的中和作用。