Genome-wide RNA-sequencing differences in nucleus accumbens of smokers vs. nonsmokers

Cigarette smoking remains the leading cause of preventable death worldwide. Cigarette smoking behaviors (e.g., initiation, nicotine dependence, cessation) are heritable, and many genetic risk loci have been identified. However, the neurobiological mechanisms underlying the genetic risk loci along the trajectory of smoking are largely unknown. RNA-sequencing (RNA-seq) differences between smokers and nonsmokers can provide insight into mechanisms that predispose to smoking behaviors and consequences of the smoking exposure itself. Here, we provide RNA-seq data generated in nucleus accumbens, an addiction-relevant brain tissue, from 223 deceased individuals: 50 current cigarette smokers, 171 nonsmokers, and 2 individuals with undetermined smoking status. DNA methylation data on the Illumina HumanMethylationEPIC array are also available on most of these individuals and made available (GEO accession number GSE147040). RNA-seq data were measured in postmortem nucleus accumbens bulk tissue. The dataset includes 223 deceased individuals, including 50 current cigarette smokers, 171 nonsmokers, and 2 individuals with undetermined smoking status.

吸烟仍是全球范围内可预防死亡的首要诱因。吸烟行为（例如吸烟起始、尼古丁依赖、戒烟）具有遗传易感性，众多遗传风险位点已被鉴定。然而，沿吸烟轨迹发挥作用的遗传风险位点背后的神经生物学机制，在很大程度上仍未明确。吸烟者与非吸烟者之间的RNA测序（RNA-sequencing, RNA-seq）差异，可为阐明吸烟行为易感机制以及吸烟暴露本身所带来的后果提供重要洞见。本数据集提供了来自223名已故个体的伏隔核（成瘾相关脑组织）的RNA测序数据：其中50名为当前吸烟者，171名为非吸烟者，另有2名吸烟状态未明确的个体。大部分受试者的DNA甲基化数据采用Illumina HumanMethylationEPIC芯片完成检测，相关数据亦已公开（基因表达综合数据库（Gene Expression Omnibus, GEO）收录编号GSE147040）。RNA测序数据来源于死后伏隔核的大块组织样本。本数据集共包含223名已故个体，其中50名为当前吸烟者，171名为非吸烟者，另有2名吸烟状态未明确的个体。