Additional file 1: of Long non-coding RNAs of switchgrass (Panicum virgatum L.) in multiple dehydration stresses

Figure S1. Overview of the RNA-Seq results and repeatability of different biological replicates. The first eight figures show the proportion of clean reads in the sequenced samples; the next three figures show the repeated expression of genes in two biological replicates; the last eight figures showed the correlations between differentially expressed genes in two biological replicates, and the distributions of upregulated and downregulated genes/lncRNAs in multiple dehydration stresses. C, control; D1–2, the first and second dehydration stresses; R1, the first recovery period. Figure S2. Water loss and survive rates in multiple dehydration stresses. A. Water loss from leaves during the first, second and third dehydration stresses. B. Survival rates of trained and non-trained switchgrass. Figure S3. Verification of four types of dehydration memory genes by quantitative real-time PCR. C, control; D1–2, first and second dehydration stresses; R1, first recovery period. Relative gene expressed levels were calculated using the ΔΔCT method with PveEF-1α as the internal control, and three biological replicates were performed for each experiment. (ZIP 1905 kb)补充图S1. RNA测序（RNA-Seq）结果概览与不同生物学重复（biological replicates）的重复性。前八幅子图展示测序样本中清洁读段（clean reads）的占比；接下来的三幅子图呈现两个生物学重复间的基因表达重复性；最后八幅子图则展示了两个生物学重复中差异表达基因（differentially expressed genes）的相关性，以及多重脱水胁迫（dehydration stresses）下上调基因、下调基因/长链非编码RNA（lncRNAs）的分布情况。C为对照组；D1~2为第1、2次脱水胁迫；R1为第1个恢复周期。补充图S2. 多重脱水胁迫下的叶片失水量与存活率。A. 第1、2、3次脱水胁迫过程中的叶片失水量；B. 驯化与未驯化柳枝稷的存活率。补充图S3. 通过实时定量聚合酶链式反应（quantitative real-time PCR，qRT-PCR）验证四类脱水记忆基因。C为对照组；D1~2为第1、2次脱水胁迫；R1为第1个恢复周期。以PveEF-1α作为内参基因，采用ΔΔCT法计算相对基因表达水平，每组实验均设置3次生物学重复。（压缩包大小1905 kb）