Daidzin Target mutant sequence reads

Targeted gene editing and gene deletion strains of Phytophthora capsici were generated for genes encoding putative daidzin receptor proteins using CRISPR-Cas9 and homologous recombination strategies. High-quality genomic DNA was isolated from individual mutant and control strains and used for Illumina-based second-generation sequencing. The resulting reads data were used for comparative analyses to confirm targeted gene deletions or edits, to examine edited genomic regions, and to assess potential off-target effects associated with the guide RNAs used during editing.

本研究针对辣椒疫霉（Phytophthora capsici）中编码推定大豆苷受体蛋白的基因，采用CRISPR-Cas9与同源重组策略，构建了靶向基因编辑及基因缺失菌株。从各突变株与对照菌株中提取高质量基因组DNA，用于基于Illumina平台的第二代测序（second-generation sequencing）。所得测序读段数据用于比较基因组分析，以验证靶向基因缺失或编辑事件、检测编辑后的基因组区域，并评估编辑过程中使用的向导RNA（guide RNAs）潜在脱靶效应。