Identification of atheroprone shear stress responsive regulatory elements in endothelial cells

While variations in gene transcription networks in models of atherosclerosis have been reported, the underlying changes in the chromatin landscape induced by pro-atherogenic stimuli remain elusive. In the present study, we report changes in chromatin regulatory elements that mediate transcriptional control upon the application of oscillatory shear stress of ±3 dyn/cm2 in primary cultured human umbilical vein endothelial cells (HUVECs) at 6 h time point of oscillatory shear stress stimulation compared to static condition. H3K27ac ChIP-Seq and total RNA-Seq profiles of HUVEC under oscillatory and static conditions were generated by paired-end sequencing using Illumina NextSeq 500 System.

尽管已有研究报道动脉粥样硬化模型中的基因转录网络存在变异，但致动脉粥样硬化刺激所诱导的染色质景观的潜在变化仍尚未明确。本研究中，我们报道了在±3 dyn/cm²的振荡剪切应力刺激原代培养人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVECs）6小时后，介导转录调控的染色质调控元件的变化，并以静态培养条件作为对照。本研究采用Illumina NextSeq 500系统进行双端测序，获取了振荡剪切应力与静态培养条件下人脐静脉内皮细胞的H3K27ac染色质免疫共沉淀测序（ChIP-Seq）及总RNA测序（RNA-Seq）图谱。