Pellicle Stimulon in Mycobacterium tuberculosis. Mycobacterium tuberculosis

Triplicate 10mL cultures of M. tuberculosis grown in Sautons media without detergent in planktonic (exponential growth phase at OD600 0.2) compared to pellicle (3 weeks growth without shaking in 5% CO2) conditions. Overall design: comparison of pellicle growth transcriptome to planktonic growth transcriptome, 3 biological replicates, third is dye-flip

将在无去污剂的索顿培养基（Sautons media）中培养至光密度OD600为0.2的指数生长期浮游态的三份10mL结核分枝杆菌（Mycobacterium tuberculosis，M. tuberculosis）培养物，与菌膜（pellicle）培养条件下的培养物进行对比。菌膜培养条件为在5%二氧化碳浓度下静置培养3周。实验整体设计：对比菌膜生长与浮游生长的转录组，共设置3组生物学重复，其中第三组为染料翻转（dye-flip）实验。