WHO-recommended hand rub formulations.

Despite continued outbreaks of yellow fever virus (YFV) in endemic regions, data on its environmental stability or guidelines for its effective inactivation is limited. Here, we evaluated the susceptibility of the YFV 17D vaccine strain to inactivation by ethanol, 2-propanol, World Health Organization (WHO)-recommended hand rub formulations I and II, as well as surface disinfectants. In addition, two pathogenic strains were tested to compare inactivation kinetics by WHO-recommended hand rub formulations I and II. Furthermore, environmental stability of the vaccine strain was assessed. YFV 17D particles displayed infectivity half-life decay profiles of ~13 days at room temperature. Despite this extended environmental stability, YFV was efficiently inactivated by alcohols, WHO-recommended hand formulations, and four out of five tested surface disinfectants. These results are useful in defining disinfection protocols to prevent non-vector borne YFV transmission.

尽管黄热病毒（Yellow Fever Virus, YFV）在流行区域仍持续暴发，但关于其环境稳定性的相关数据，以及有效灭活的指导方案均较为匮乏。本研究评估了YFV 17D疫苗株对乙醇、异丙醇（2-propanol）、世界卫生组织（World Health Organization, WHO）推荐的手消毒剂配方I与II，以及表面消毒剂的灭活敏感性。此外，本研究还测试了两株致病性黄热病毒毒株，以对比世界卫生组织推荐的手消毒剂配方I与II的灭活动力学特性；同时评估了该疫苗株的环境稳定性。实验结果显示，YFV 17D病毒粒子在室温条件下的感染性半衰期约为13天。尽管该病毒具备较好的环境稳定性，但乙醇、异丙醇、世界卫生组织推荐的手消毒剂配方，以及5种受试表面消毒剂中的4种，均可高效灭活YFV。本研究结果可为制定预防非媒介传播黄热病毒的消毒方案提供重要参考依据。