Expression of long non-coding RNAs during spermatogenesis. Mus musculus

Total RNA from purified stages of spermatogenesis were subjected to next generation RNA-seq (100bp, paired-end, strand-specific). The expression of mRNAs and lncRNAs was calculated as FPKM values. Overall design: Three biological replicates of spermatogonia, pachytene and round spermatids were utilized for RNA isolation.

将纯化获得的精子发生各阶段样本的总RNA进行下一代RNA测序（100bp读长、双端、链特异性）。以FPKM值计算信使RNA（messenger RNA, mRNA）与长链非编码RNA（long non-coding RNA, lncRNA）的表达水平。实验设计概述：本研究选取精原细胞、粗线期精母细胞及圆形精子细胞各三个生物学重复样本用于RNA提取。