Original code: Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries

Original code related to Cardona et al., Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries.

This repository contains original code and example data to perform:

- fluorescent reporters quantification
- smFISH images simulation
- smFISH images quantification
- nanoclusters fitting
- condensate quantification
- translation rates computing

Each folder contains a README.md and a .pdf file explaining how to use the respective code.

Source:
https://github.com/CardonaEA/code-image-analysis-c-elegans
DOI: https://doi.org/10.5281/zenodo.8221981

Reference
Cardona et al., Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries, Cell (2023), https://doi.org/10.1016/j.cell.2023.08.018

本代码与Cardona等人的研究论文《mRNA拷贝的自分离效应可缓冲mRNA:mRNA与mRNA:调控因子的化学计量比》(Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries)相关。
本仓库包含用于开展以下分析的原始代码与示例数据集：
- 荧光报告因子定量
- 单分子荧光原位杂交(smFISH)图像模拟
- 单分子荧光原位杂交(smFISH)图像定量
- 纳米团簇拟合
- 凝聚体定量
- 翻译速率计算

每个子文件夹均附带README.md文档与.pdf说明文件，详细介绍对应代码的使用方法。

来源：
https://github.com/CardonaEA/code-image-analysis-c-elegans
DOI: https://doi.org/10.5281/zenodo.8221981

参考文献：
Cardona et al., Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries, *Cell* (2023), https://doi.org/10.1016/j.cell.2023.08.018