Original code: Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries

Original code related to Cardona et al., Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries. This repository contains original code and example data to perform: - fluorescent reporters quantification - smFISH images simulation - smFISH images quantification - nanoclusters fitting - condensate quantification - translation rates computing Each folder contains a README.md and a .pdf file explaining how to use the respective code. Source: https://github.com/CardonaEA/code-image-analysis-c-elegans DOI: https://doi.org/10.5281/zenodo.8221981 Reference Cardona et al., Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries, Cell (2023), https://doi.org/10.1016/j.cell.2023.08.018本代码与Cardona等人的研究论文《mRNA拷贝的自分离效应可缓冲mRNA:mRNA与mRNA:调控因子的化学计量比》(Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries)相关。 本仓库包含用于开展以下分析的原始代码与示例数据集： - 荧光报告因子定量 - 单分子荧光原位杂交(smFISH)图像模拟 - 单分子荧光原位杂交(smFISH)图像定量 - 纳米团簇拟合 - 凝聚体定量 - 翻译速率计算 每个子文件夹均附带README.md文档与.pdf说明文件，详细介绍对应代码的使用方法。 来源： https://github.com/CardonaEA/code-image-analysis-c-elegans DOI: https://doi.org/10.5281/zenodo.8221981 参考文献： Cardona et al., Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries, *Cell* (2023), https://doi.org/10.1016/j.cell.2023.08.018