RIPSeq analysis (CoIP) of CcaF1 from R. sphaeroides 2.4.1 under phototrophic growth conditions

In previous studies we identified the small RNA-binding protein CcaF1 that is involved in sRNA maturation and RNA turnover in Rhodobacter sphaeroides under microaerobic growth conditions (Grützner et al., 2021, Nucleic Acids Res. 49(6), doi: 10.1093/nar/gkab146). In this study we analysed the the small RNA-binding protein CcaF1 under phototrophic growth condtions to identify new RNA binding partners. Overall design: The CcaF1 protein was overexpressed with an 3xFLAG (pRKccaF1FLAG) in R. sphaeroides under phototrophic growth condtions

前期研究中，我们在微好氧生长条件下的球形红杆菌（Rhodobacter sphaeroides）中鉴定出一种参与sRNA（small RNA）成熟与RNA降解的小RNA结合蛋白CcaF1（Grüutzner等人，2021，《核酸研究》（Nucleic Acids Research），49(6)，doi: 10.1093/nar/gkab146）。本研究针对光养生长条件下的小RNA结合蛋白CcaF1开展分析，以鉴定其新型RNA结合伴侣。实验整体设计：将带有3×FLAG标签的CcaF1蛋白（质粒pRKccaF1FLAG）在光养生长条件下的球形红杆菌（R. sphaeroides）中进行过表达。