HFD-induced transcriptomic profile of mouse brain cortices in APOE3 and APOE4 background. Mus musculus strain:C57BL/6

This work investigated the effect of High-fat diet (HFD) on mouse brain cortices, in APOE3 and APOE4 background. We hypothesized that HFD-induced obesity alters brain transcriptomics in APOE-genotype and sex dependent manners. Six-month-old APOE3-TR and APOE4-TR mice were treated with either HFD or control chow. After 4 months, total RNA was extracted from the cerebral cortices and analyzed by poly-A enriched RNA sequencing on the Illumina platform. Here, we investigated interactions between HFD, APOE, and sex, using a knock-in mouse model of the human APOE3 and APOE4 alleles.

本研究探究了高脂饮食（high-fat diet, HFD）对载脂蛋白E3（APOE3）与载脂蛋白E4（APOE4）遗传背景下小鼠大脑皮层的影响。我们提出假设：高脂饮食诱导的肥胖会以APOE基因型和性别依赖的方式改变大脑转录组特征。选取6月龄的APOE3靶向替换（APOE3-TR）与APOE4靶向替换（APOE4-TR）小鼠，分别给予高脂饮食或对照饲料干预。干预4个月后，提取小鼠大脑皮层的总RNA，并通过聚腺苷酸富集RNA测序（poly-A enriched RNA sequencing）在Illumina测序平台完成分析。本研究借助人源APOE3与APOE4等位基因的敲入小鼠模型，探究了高脂饮食、APOE基因型与性别三者间的交互作用。