Replication Data for: The impact of wheat sprouting on iron and zinc bioaccessibility and bioavailability to Caco-2 cells

While wheat is a good source of iron (Fe) and zinc (Zn), their bioaccessibility is limited due to chelation with phytic acid. Sprouting wheat at 26 °C reduced the phytate content by 25 – 40 % and increased bioaccessibility (determined with an in vitro digestion assay) 1.5 – 2.7 times (Fe) and 1.6 – 2.3 times (Zn), depending on the sprouting time (48 – 120 h). Subsequent application of in vitro digests to Caco-2 cells showed that sprouting for 120 h at 26 °C did not enhance wheat Fe bioavailability, while it increased Zn bioavailability 1.6-fold. When the in vitro digests were exposed to diffusive gradients in thin film (DGT) with Chelex resin, which binds free mineral ions, sprouting was found to have caused release of non-labile Fe complexes and some labile Zn complexes. The latter resulted in higher levels of bioavailable forms of Zn in wheat.

尽管小麦是铁（Fe）与锌（Zn）的优质膳食来源，但二者的生物可及性（bioaccessibility）因与植酸（phytic acid）发生螯合作用而受到限制。在26℃条件下对小麦进行发芽处理，可使植酸含量降低25%~40%，并提升其生物可及性——该指标通过体外消化试验（in vitro digestion assay）测定——铁与锌的生物可及性分别提升1.5~2.7倍与1.6~2.3倍，提升幅度取决于发芽时长（48~120 h）。后续将体外消化产物施加于Caco-2细胞的实验显示，26℃下发芽120 h并未提升小麦中铁的生物利用度（bioavailability），却使锌的生物利用度提升1.6倍。当将体外消化产物与负载Chelex树脂（Chelex resin）的薄膜扩散梯度（diffusive gradients in thin film, DGT）装置（该装置可结合游离矿物离子）接触时，研究发现发芽处理会促使非活性铁复合物与部分活性锌复合物释放，其中活性锌复合物使得小麦中可被生物利用的锌含量进一步升高。