3D spheroid human placenta-derived mesenchymal stem cells enhances anti-inflammatory response and trophic-factors secretion

We found significant difference between the 3D-spheroid cultured- and conventionally cultured mesenchymal stem cells (3D MSCs and 2D MSCs) in terms of anti-inflammatory response and ability to secrete trophic factors, implying that 3D MSCs may better improve the tissue repair and promote functional recovery. Next-generation sequencing has revolutionized system-based analysis of cellular pathways. The aims of this study are to compare the transcriptome profile between the 3D-spheroid cultured- and conventionally cultured mesenchymal stem cells (3D MSCs and 2D MSCs). Overall design: Human placenta derived MSCs (HuMSCs) were isolated and cultured for three passages. Then they were cultured as 3D MSCs and 2D MSCs, respectively. Two types of cells were collected and prepared for the RNA-sequencing.

本研究发现，3D球体培养的间充质干细胞(mesenchymal stem cells, MSCs)与常规培养的间充质干细胞（3D MSCs、2D MSCs）在抗炎反应及分泌营养因子的能力方面存在显著差异，提示3D MSCs或可更有效地改善组织修复并促进功能恢复。下一代测序(next-generation sequencing)已彻底革新了基于系统的细胞通路分析。本研究旨在比较3D球体培养与常规培养的间充质干细胞（3D MSCs与2D MSCs）的转录组谱。实验设计：分离人胎盘来源间充质干细胞（human placenta derived MSCs, HuMSCs）并传代培养3次，随后将其分别诱导为3D MSCs与2D MSCs，收集两类细胞并制备样本用于RNA测序(RNA-sequencing)。