Identification of immune signatures associated with both SARS-CoV-2 infection and lung transplantation

We used single-cell RNA sequencing to analyze immune cells in COVID-19 patients. We drew blood from convalescent patients and examined PBMCs, monocytes and regulatory T cells. Monocytes and Tregs were sorted using fluorescence activated sorting (FACS). All samples were sequenced using a 10X genomics library preparation kit and an Illumina HiSeq sequencer.

本研究借助单细胞RNA测序（single-cell RNA sequencing）技术，针对新型冠状病毒肺炎（COVID-19）患者体内的免疫细胞开展分析。我们从康复期患者体内采集血液样本，对外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）、单核细胞及调节性T细胞进行检测。其中单核细胞与调节性T细胞（Tregs）通过荧光激活细胞分选术（fluorescence activated sorting, FACS）完成分选。所有样本均采用10X Genomics文库制备试剂盒与Illumina HiSeq测序仪完成测序。