Genome-wide maps of ARID1A binding genes in H9 human embryonic stem cells

We reported loss of ARID1A promoted neurogenesis and inhibited cardiogenesis. Here we used specific ARID1A antibody to pull down ARID1A binding genomic DNA in human embryonic stem cells, which let us know the potential genes regulated by ARID1A during neurogenesis and inhibited cardiogenesis. IgG was used as the control. Overall design: Examination of ARID1A binding genes in hESCs.

本研究此前报道了AT富集互作结构域蛋白1A（ARID1A）缺失可促进神经发生并抑制心脏发生。本实验通过在人类胚胎干细胞（human embryonic stem cells, hESCs）中使用特异性ARID1A抗体免疫沉淀富集ARID1A结合的基因组DNA，以明确ARID1A在促进神经发生及抑制心脏发生过程中调控的潜在靶基因；实验以免疫球蛋白G（Immunoglobulin G, IgG）作为对照。整体实验设计：检测人类胚胎干细胞中ARID1A结合的基因。