Comparison between all runs of industrial serial repitching 3

The aim of this study was to identify differently expressed genes between all runs of four serial repitchings from a German brewery. In the brewery the number of successive runs varied from 17 to 20. A run describes the industrial fermentation of brewery wort. By the end of fermentation the yeast slurry is cropped. These cells are reused for inoculation of another cylindro-conical vessel (CCVs). Results from four loops are summarized in this study. The samples were taken from four serial repitchings of a German brewery (brewery A). Microarrays were hybridized in a loop approach.

本研究旨在鉴定某德国啤酒厂四次连续酵母复投过程中所有发酵批次（run）间的差异表达基因。该啤酒厂的连续发酵批次数量介于17至20之间。本研究中所指的发酵批次，即啤酒麦芽汁的工业发酵流程；发酵结束后回收酵母泥，所得酵母细胞会被再次用于接种另一台圆柱锥底发酵罐（cylindro-conical vessels, CCVs）。本研究汇总了四次复投循环的实验结果，实验样本取自德国A啤酒厂的四次连续酵母复投过程，且采用循环杂交法完成基因芯片（microarrays）的杂交。