Ahringer AB8895_H3K4ME1733246_N2_L3

modENCODE_submission_2775 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: L3 Larva; Genotype: wild type; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 2; EXPERIMENTAL FACTORS: Developmental Stage L3 Larva; temperature 20; Antibody ab8895 H3K4me1:733246 (target is H3K4me1); Strain N2

modENCODE_submission_2775 本数据集提交来自Jason Lieb主持的modENCODE项目。如需查看modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。项目目标：本分析的研究重点为具备以下功能的调控元件：调控核小体定位与占据状态、控制基因表达结构域、诱导X染色体沉默、指导有丝分裂分离与基因组复制、调控减数分裂过程中同源染色体配对与重组，以及调控细胞核内的染色体定位。本次筛选得到的126个经策略性筛选的靶点涵盖关键组蛋白修饰、组蛋白变体、RNA聚合酶II同工型、剂量补偿蛋白、着丝粒组分、同源染色体配对辅助因子、重组标记物以及核膜组分。有关数据使用条款与细则，请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。实验类型：ChIP-chip。生物来源：菌株：N2；发育阶段：L3幼虫；基因型：野生型；性别：混合雌雄群体（雄性与雌雄同体）。生物学重复次数：2次。实验因素：发育阶段为L3幼虫；培养温度20℃；抗体：ab8895 H3K4me1（编号733246，靶标为H3K4me1）；菌株：N2