Phosphoproteomics analysis of Pseudomonas aeruginosa with PfkA, PfkB and PfpC overproduction

To determined the phosphorylation sites of proteins after overproduction of PfkA, PfkB and PfpC individually in PAO1 via pHERD20T-pfkA, pHERD20T-pfkB and pHERD20T-pfpC.本研究旨在鉴定在PAO1菌株中，分别通过pHERD20T-pfkA、pHERD20T-pfkB及pHERD20T-pfpC质粒过量表达PfkA、PfkB与PfpC蛋白后，蛋白质的磷酸化位点。