EHMT1 and EHMT2 inhibition induce fetal hemoglobin expression [ChIP-seq]. Homo sapiens

Using UNC0638 and genetic assays to inhibit EHMT1/2 and derepress fetal hemoglobin in adult hematopoietic cells. Overall design: ChIP-seq for 2 histone modifications and total H3 (for quantitative normalization) in biological triplicates of cord blood, adult bone marrow, and UNC0638-treated adult bone marrow.

本数据集采用UNC0638与遗传学实验手段，在成人造血细胞中抑制EHMT1/2并去抑制胎儿血红蛋白的表达。整体实验设计：对脐带血、成人骨髓以及经UNC0638处理的成人骨髓三种样本各设置三次生物学重复，分别开展针对2种组蛋白修饰及总H3蛋白（用于定量标准化）的染色质免疫共沉淀测序（ChIP-seq）实验。