Data from: Fructooligosaccharides (FOS) differentially modifies the in vitro gut microbiota in an age-dependent manner

A 24-h in vitro culturing method was used to determine whether fructooligosaccharide (FOS) treatment effects on the human gut microbiome has a differential effect by age, using fecal samples from 18 adults in 3 age groups with 6 individuals for each group, young adult (25–35 years of age), adult (36–50 years of age), and older adult (51–70 years of age). Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883714: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883714), total bacterial qPCR, Bifidobacterium genus specific q-PCR, and short chain fatty acid concentrations.

本研究采用24小时体外培养方法，旨在探究低聚果糖（fructooligosaccharide, FOS）干预对人类肠道菌群的影响是否存在年龄依赖性差异。实验纳入18名成年人的粪便样本，按年龄划分为3组，每组各6名受试者：青年组（25~35岁）、中年组（36~50岁）及老年组（51~70岁）。本研究采集的检测数据包括：16S rRNA基因V1-V2可变区扩增子测序数据（相关数据集已上传至NCBI序列读取档案（Sequence Read Archive, SRA），关联生物项目编号PRJNA883714，链接：https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883714）、总细菌定量聚合酶链反应（quantitative polymerase chain reaction, qPCR）检测结果、双歧杆菌属特异性定量聚合酶链反应（quantitative polymerase chain reaction, qPCR）检测结果，以及短链脂肪酸浓度检测结果。