Qualifying a human Liver-Chip for predictive toxicology: Performance assesment and economic implications. Qualifying a human Liver-Chip for predictive toxicology: Performance assesment and economic implications

We assess RNA expression in human primary hepatocytes in Liver-Chips on Day 3 and Day 5. The hepatocytes were co-cultured in Liver-Chip model with Kupffer cells, Liver sinusoidal endothelial cells (LSECs) and stellate cells . Overall design: Global RNA expression profiles of Liver-Chips Day3 (n=4); Global RNA expression profiles of Liver-Chips Day5 (n=4); Generated by deep sequencing using Illumina HiSeq.

本研究于第3天及第5天，对肝脏芯片（Liver-Chip）中的人类原代肝细胞开展RNA表达水平检测。实验采用肝脏芯片模型，将人类原代肝细胞与库普弗细胞（Kupffer cells）、肝窦内皮细胞（Liver sinusoidal endothelial cells，LSECs）及星状细胞共培养。实验设计概述：第3天肝脏芯片的全局RNA表达谱（样本量n=4）；第5天肝脏芯片的全局RNA表达谱（样本量n=4）；所有测序数据通过Illumina HiSeq深度测序获取。