Raw data from transcriptome sequencing of A549/DDP cells

This study employed the A549/DDP human lung adenocarcinoma cisplatin-resistant cell line, which was divided into two experimental groups according to the study design: (1) blank control group (Control); (2) β-Elemene treatment group (B). Each group included three biological replicates.The experimental procedure was as follows: A549/DDP cells were expanded and seeded into 24-well plates. Transfection complexes were prepared using Opti-MEM (500 μL), Lipofectamine 2000 (20 μL), and either LINC00511 siRNA (50 μL) or an overexpression plasmid (15 μg). Cells were transfected after overnight incubation at 37 ℃ in a 5% CO₂ atmosphere. Twenty-four hours post-transfection, cells received designated treatments according to their assigned groups: final concentrations of DDP at 24 μM and β-Elemene at 40 μg/mL. Following an additional 24-hour incubation under standard culture conditions (37 ℃, 5% CO₂), cells were harvested, washed with PBS, lysed thoroughly in TRIzol reagent, snap-frozen in liquid nitrogen, and submitted to Wuhan Lingsi Biotechnology Co., Ltd. for transcriptome sequencing. Sequencing was performed on the DNBSEQ-T7RS High-throughput Sequencing System (PE150) using chemistry version V3.0.

本研究采用人肺腺癌顺铂（DDP）耐药细胞系A549/DDP，按照实验设计分为两组：(1)空白对照组（Control）；(2)β-榄香烯处理组（B）。每组设置3个生物学重复。实验流程如下：将A549/DDP细胞扩增后接种于24孔板中。使用Opti-MEM（500 μL）、Lipofectamine 2000（20 μL），分别搭配LINC00511小干扰RNA（siRNA，50 μL）或过表达质粒（15 μg）制备转染复合物。将复合物加入细胞后，于37 ℃、5% CO₂培养箱中过夜孵育以完成转染。转染24小时后，按照分组对细胞施加对应处理：终浓度为24 μM的顺铂（DDP）以及40 μg/mL的β-榄香烯。在标准培养条件（37 ℃、5% CO₂）下继续孵育24小时后，收集细胞，用磷酸盐缓冲液（PBS）洗涤，经TRIzol试剂充分裂解后置于液氮中快速速冻，随后送至武汉灵思生物技术有限公司进行转录组测序。测序采用DNBSEQ-T7RS高通量测序系统（PE150），使用V3.0化学试剂完成测序。