RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. [Cre]. RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. [Cre]

RNA sequencing of FACS sorted microglia isolated from brain tissue of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice was performed to test for potential differences in gene expression caused by the knock-in of tdTomato and CreERT2 into the Hexb locus. Overall design: Microglia were isolated from brain tissue and RNA expression levels between individual samples were compared.

为检测将tdTomato与CreERT2敲入Hexb基因座所引发的潜在基因表达差异，本研究对取自野生型（WT）、Hexb-tdTomato、Hexb-CreERT2及Hexb-KO小鼠脑组织的、经荧光激活细胞分选（FACS, Fluorescence-Activated Cell Sorting）纯化的小胶质细胞开展了RNA测序实验。实验整体设计：从脑组织中分离小胶质细胞，对各独立样本的RNA表达水平进行比较分析。