Expression data from human astrocytes (HA) under oxygen-glucose deprivation / reoxygenation

Ischemia lead to neuronal injury. Dexmedetomidine and astrocytes are likely to protect neuronal cells against ischemia-induced injury. We used microarrays to examine the gene expression variation in astrocytes activated by oxygen-glucose deprivation and reoxygenation stress and identified distinct classes of genes up- and down-regulated by dexmedetomidine pretreatment. Human astrocytes were subjected to oxygen glucose deprivation and reoxygenation (OGD/R) stress for RNA extraction and hybridization on Affymetrix microarrays. To exam the preventive effect of DEX, cells were cultured with DEX for 1 hour, before OGD/R. Cells were washed by PBS and replaced with a glucose-free medium, set in a sealed container, filled with 95% N2 / 5% CO2, and incubated for 4 hours. The cells were then returned to air conditions, washed out and replaced with normal medium, and cells were incubated for a constant reoxygenation time. i.e., 0 h (HAC 0h and HAD 0h), 2 h (HAC 2h and HAD 2h), 6 h (HAC 6h and HAD 6h), 12 h (HAC 12h and HAD 12h), and 24 h (HAC 24h and HAD 24h)

缺血可引发神经元损伤。右美托咪定（Dexmedetomidine）与星形胶质细胞或可保护神经元细胞免受缺血诱导的损伤。我们采用微阵列芯片（microarray）技术检测氧糖剥夺/复氧应激激活的星形胶质细胞中的基因表达差异，并筛选得到经右美托咪定预处理后显著上调与下调的不同基因类别。
我们将人星形胶质细胞暴露于氧糖剥夺/复氧（OGD/R）应激环境中，随后提取RNA并在Affymetrix微阵列芯片上进行杂交反应。为验证右美托咪定（DEX）的预防作用，我们在氧糖剥夺/复氧应激处理前1小时，将细胞置于含右美托咪定的培养基中培养。随后用磷酸盐缓冲液（PBS）洗涤细胞，更换为无糖培养基，将细胞置于充有95%氮气与5%二氧化碳的密封容器内孵育4小时。随后将细胞恢复至正常空气培养环境，洗涤后更换为正常培养基，按照固定时长进行复氧培养，即复氧时长分别为0小时（HAC 0h、HAD 0h）、2小时（HAC 2h、HAD 2h）、6小时（HAC 6h、HAD 6h）、12小时（HAC 12h、HAD 12h）以及24小时（HAC 24h、HAD 24h）