Study the differential gene expression of C57/BL6 Mouse tumors treated with EZH2 inhibitor GSK343 II

The study evaluated the differential gene expression in control Antibody or Ant-CXCL10 antibody injected mouse when compared to EZH2 inhibitor GSK343 treated mice tumors Briefly, control Antibody or Ant-CXCL10 antibody injected C57BL/6 mouse were treated with or without GSK343were analyzed for RNA-seq Total RNA was extracted from C57/BL6 mouse tumors, followed by poly-A selection and RNA-sequencing using Illumina Hi-Seq 2500 platform, with three biological replicates for each transformed sample.

本研究对比分析了注射对照抗体或抗CXCL10抗体（Ant-CXCL10 antibody）的小鼠，与经EZH2抑制剂（EZH2 inhibitor）GSK343处理的小鼠肿瘤中的差异基因表达水平。简要而言，实验选取注射了对照抗体或抗CXCL10抗体的C57BL/6小鼠（C57BL/6 mouse），对其分别施以GSK343处理与空白对照，随后开展RNA测序（RNA-seq）分析：实验步骤为先从C57BL/6小鼠的肿瘤组织中提取总RNA，经polyA富集（poly-A selection）后，使用Illumina Hi-Seq 2500测序平台进行RNA测序；每个处理组均设置3个生物学重复（biological replicates）。