DAB stainings of human liver samples with the markers used in immunofluorescence multiplexing

By integrating single-nucleus transcriptomics, spatial multi-omics, and proteomics in human liver samples, we delineate the evolving landscape of hepatic macrophages across the MASLD spectrum. Attached presentation include representative images of standard immunohistochemistry (DAB) on liver samples from at-risk MASH patients showing the expression of macrophage markers used in immunofluorescence multiplexing.

本研究通过在人类肝脏样本中整合单细胞核转录组学（single-nucleus transcriptomics）、空间多组学（spatial multi-omics）与蛋白质组学（proteomics），刻画了代谢相关脂肪性肝病（MASLD）谱系中肝巨噬细胞的动态变化图谱。本数据集随附的演示材料包含了高危代谢相关脂肪性肝炎（MASH）患者肝脏样本的标准免疫组织化学（DAB）代表性图像，该图像展示了多重免疫荧光实验中所用巨噬细胞标志物的表达情况。