Data_Sheet_1_Identification of a Newly Conserved SLA-II Epitope in a Structural Protein of Swine Influenza Virus.PDF

Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs.

尽管猪作为潜在可引发人类大流行的新型甲型流感病毒（Influenza A Virus, IAV）的宿主，但其针对猪流感病毒（swine influenza virus, SwIV）的免疫应答尚未完全阐明。此前已有多项研究通过不同方法，在远交猪或经全基因组单核苷酸多态性（single nucleotide polymorphism, SNP）分析显示基因组同源性达85%的巴布拉克大白近交系猪中，鉴定出了由猪主要组织相容性复合体（swine leukocyte antigen, SLA）I类分子呈递的多个SwIV表位。另一方面，近期在远交猪中也报道了若干SLA II类表位。本研究选用巴布拉克大白近交系猪，以鉴定猪流感病毒H1N1的SLA II类表位：通过干扰素γ酶联免疫斑点试验（IFNγ ELISPOT），筛选可识别覆盖异源IAV H1N1核蛋白（nucleoprotein, NP）与基质蛋白1（matrix protein 1, M1）的重叠肽段的外周血单个核细胞（peripheral blood mononuclear cell, PBMCs），最终在猪H1N1的NP蛋白中鉴定出一个新型SLA II类限制性表位。该保守新型表位可作为猪群抗H1N1流感疫苗研发的潜在基础。