Suppl Figure S3 secondary structure pilA-C.docx

Supplementary Figure S3. Secondary structure of “PilA-C” proteins from Desulfuromondales species and other bacteria that possess e-pilin genes. The location of alpha helices (red H) and beta strands (yellow E) was predicted with Jpred 4 (Drozdetskiy et al, 2015). Signal peptides (green background) were identified with PSORTb v. 3.0.2 (Yu et al., 2010) and SignalP v. 4.1 (Petersen et al., 2011). Transmembrane helices (turquoise background) were predicted with TmPred (Hofmann & Stoffel, 1993), TMHMM (Krogh et al, 2001), and HMMTOP (Tusnady & Simon, 2001).

补充图S3：来自脱硫单胞菌目（Desulfuromondales）物种及其他携带e-菌毛蛋白（e-pilin）基因的细菌的"PilA-C"蛋白二级结构。α螺旋（红色标记为H）与β折叠（黄色标记为E）的定位通过Jpred 4（Drozdetskiy等，2015）预测得到。信号肽（以绿色背景标注）通过PSORTb v.3.0.2（Yu等，2010）与SignalP v.4.1（Petersen等，2011）完成鉴定。跨膜螺旋（以青绿色背景标注）通过TmPred（Hofmann与Stoffel，1993）、TMHMM（Krogh等，2001）以及HMMTOP（Tusnady与Simon，2001）预测得到。